Lost Souls can refer to several things:
- Lost Souls is a 2000 movie directed by Janusz KamiÅ„ski and starring Winona Ryder and Elias Koteas
- "Lost Souls" is a 1997 miniseries created by Matthew Schutt.
- Lost Souls (1992) is the debut novel of horror author Poppy Z. Brite
- Lost Souls is the 2000 debut album by British band Doves.
- Lost Souls is an expansion to the computer game, Earth 2150.
- Lost Souls is a heavy metal band.
- A Lost Soul is a monster from the Doom series of computer games.