"Neo" may mean:
* Neo-, a prefix signaling a "New" form or a revival of an old one.
* Neo (The Matrix), the protagonist in the Matrix series.
* Neo (constructed language), a constructed language.
* Neo (magazine) a UK anime, manga and Asian entertainment magazine.
* Neo (keyboard), a portable keyboard from AlphaSmart.
* Neo (band), an Indonesian hip hop vocal group.
* Dr. Neo Cortex, from the Crash Bandicoot series.
* Neo Roanoke the brainwashed alter-ego of Mwu La Flaga from Gundam Seed Destiny
* An abbreviation for neoprene, often used by scuba divers when referring to exposure suits.
* Neo-soul, a style of music
* Neopets, an online virtual pets game.
* Neo (object-relational toolset), a programming framework for use with the .NET Framework.
* Neo (musician), producer behind Belgian electronic music act Infrabuse.
"NEO" may stand for:
* Near-Earth object
* Neuroticism Extraversion Openness Personality Inventory, or ''NEO PI-R'', a psyschological personality inventory.
* NEO (keyboard layout) - http://pebbles.schattenlauf.de/layout/index_us.html
* Northeast Ohio - the most populous area of the state, anchored by Cleveland