Highway 51 Blues Bob Dylan {c:Played in Open D/E tuning. The song is played in E, so either tune in open E or (recommended) in open D with a capo on the 2nd fret. The basic chords are: {define: E 000000 {define: A 020120 {define: B x02100 {c:The most dominant riff that goes through the whole song, or rather: wherever there is an E chord, is (it is mainly played on the bass strings, but of course it doesn't matter if more strings are sounding): {sot : . . . : . . . |-0---------------|------- (dylanchords.info)